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. Author manuscript; available in PMC: 2016 Dec 9.
Published in final edited form as: J Neurochem. 2012 Apr 27;122(2):321–332. doi: 10.1111/j.1471-4159.2012.07754.x

Figure 1. IVIg protects cultured neurons against simulated ischemia.

Figure 1

a. Treatment with low concentrations of IVIg (0.1 mg/ml, 0.3 mg/ml) significantly increased GD-induced neuronal cell death, whereas high concentrations of IVIg (3 mg/mL, 5 mg/mL, 10 mg/mL) significantly decreased GD-induced neuronal cell death. ***, P < 0.05 compared to the vehicle + GD group; +++, P < 0.05 compared to the GD group. b. Treatment with high concentrations of IVIg (5 mg/mL, 10 mg/mL) also decreased OGD-induced neuronal cell death. ***, P < 0.05 compared to the normal group; +++, P < 0.05 compared to the vehicle + OGD group. Treatment with a low concentration of IVIg (0.1 mg/mL) increased the expression level of cleaved caspase 3 (c and d), whereas high concentrations of IVIg (2.5 mg/mL, 5 mg/mL) significantly reduced the expression level of cleaved caspase-3 (D) in primary neuronal cells subjected to GD conditions for 12 h. *, P < 0.05 compared to GD + vehicle group; ++, P < 0.01 compared to GD + vehicle. e and f. Similarly, the level of cleaved caspase-3 increased with low concentrations of IVIg (0.1 mg/mL) and decreased with high concentrations of IVIg treatment (2.5 mg/mL, 5 mg/mL) in primary neuronal cells subjected to OGD conditions for 12 h. n = 3 or more biologically different cultures in each group. *, P < 0.05 compared to GD + vehicle group; ++, P < 0.01 compared to GD + vehicle.