Table 1.
Twenty-six pairs of PCR primers used in this study
| Gene | Number of primer | Forward primer (5’→3’) | Reverse primer (5’→3’) |
|---|---|---|---|
| NCSTN | Primer-1 | AAACACGAACTTCCGGTCTC | GAACTTCTGTCGTGGGAACG |
| Primer-2 | CCTTTGAGGCACATAGCTGG | TGCCTAGCTTGACAGACGG | |
| Primer-3 | AGAGGTGCCTGTATTCACCC | TTCCTTCTGAGGACCACCC | |
| Primer-4 | AGAGCAGATCATTGTCCAGACTC | AGGAGAGGGAAGGGATGAGC | |
| Primer-5 | CCCCTTCCTTTGCCTTAC | CACTGAAACCTCTGCCTC | |
| Primer-6 | ACTGAGTCTGCAACCCTTTG | CAGAGCTCCTTCATGGTGTC | |
| Primer-7 | TGGATAGTGGCAGAGAAGCC | CCTCCTTTCTTGCTCCAGTC | |
| Primer-8 | AAATTGGGAAGCCTCAAATG | CACATCCAGAAGCTCTGTGC | |
| Primer-9–10 | GATAGTCACAGCTGGGAGGTG | ACACCCAATCCAATCCTGTC | |
| Primer-11 | ATTCAGAGAGCCTTGGTCCC | GGACCTGAAGTTCTGGAGGG | |
| Primer-12–13 | ACCACCTCACCCTCACTCC | ATAAGATGCAGATGAGGCCC | |
| Primer-14–15 | CTTGCCCTAGTGTCCCAAAC | CTGCATCTCTCCACCTCTCC | |
| Primer-16 | GGAGAGGTGGAGAGATGCAG | GGGACAGATTTGCAGTAGGC | |
| Primer-17 | TAGGCTGGAGAGATGTTGCC | GGAGCGGAGGAAGGGAG | |
| PSENEN | Primer-2 | AGCTCTTTAATCCAGCCAGC | GTTCGCAGGTCCTTCATCTC |
| Primer-3–4 | ATTCCTGGATCCCAAAGAGG | GTCAGCAGAGAACGTGGGAC | |
| PSEN1 | Primer-3 | CCTTTGCGGTCCTTAGACAG | GTGTCCTCCAGCAATCAGC |
| Primer-4 | CAGAGAGAATGGAGCAAGCC | ATCACAGAGGATGGGCTCTC | |
| Primer-5 | ATGGAGCCAGTGTCTGCTTC | AGCTGCTTGTGAACTCCTGG | |
| Primer-6 | ACCAATATCTAGGTAAAGCCATTC | TTATAAGCAAGGAGCAACAGAAG | |
| Primer-7 | AATAGCACAGTTGATATAGGTTATGG | TGAAGAGTTATGGGATGTACACG | |
| Primer-8 | CAGTTCACCTGCCATTTATTTC | AGATCTGCAGGAGTTCCAGG | |
| Primer-9 | GCAGCATTAGGAAGACTGGC | CCTTTCCATGCTGGTATTCTG | |
| Primer-10 | AGAGGCTTGGTGGGATTACC | CCAGGTACAGTGGCTCACG | |
| Primer-11 | AACAGCAGCATCTACAGTTAAGAC | GCTGGAATATTTAACCCACCTG | |
| Primer-12 | TTCCAGATTGAATGAACGTCTG | AGTGCAAGGTGGTCAGGAAG |
PCR: Polymerase chain reaction.