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. 2016 Dec 9;7:183. doi: 10.1186/s13287-016-0447-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Self-assembly methods for cartilage formation by human mesenchymal stem cells (hMSCs). a Top, disc culture: hMSCs were seeded in transwell inserts coated with vehicle control (Veh), type I collagen (Col1), or type II collagen (Col2); Veh group failed to form discs. Bottom, pellet culture: hMSCs were seeded in round-bottom wells and formed spherical aggregates. b Representative images of hyaline discs formed in coated transwells are shown