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. 2016 Dec 8;6:43. doi: 10.1186/s13395-016-0115-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Representative images of primary and immortalized human myotubes. Myogenic cells were purified by magnetic bead sorting of CD56 expression then differentiated for 5 days. Examples here are from primary (a) and immortalized (b) populations of a healthy subject (CHQ). Myotubes were immunostained for desmin (green) to determine the percentage of myogenic purity (>94% for all samples). Magnified region shows the structure of desmin filaments in both primary and immortalized cells. Nuclei are DAPI-stained (blue). Scale = 100 μm