| Strains |
|
|
|
E. coli DH5α |
F− φ80d lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1hsdR17 (rk− mk+) supE44 λ−thi-1 gyrA relA1
|
[37] |
|
E. coli BL21 (DE3) |
F−
ompT hsdSB (rB−mB−) gal dcm met(DE3) |
Novagen |
|
C. jejuni 81116 (NCTC 11828) |
Wild-type |
[38] |
|
C. jejuni 81116 ΔtsdA
|
Kanr; insertion of kanamycin resistance cassette in place of c8j_0815
|
[2] |
|
C. jejuni 81116 ΔtsdA/*tsdA wt |
Kanr, Cmr; ΔtsdA mutant complented with c8j_0815 in cj0046 pseudogene locus behind fdxA promotor |
The present study |
|
C. jejuni 81116 ΔtsdA/*tsdA CG |
Kanr, Cmr; ΔtsdA mutant complented with tsdA C138G in cj0046 pseudogene locus behind fdxA promotor |
The present study |
|
C. jejuni 81116 ΔtsdA/*tsdA MG |
Kanr, Cmr; ΔtsdA mutant complented with tsdA M255G in cj0046 pseudogene locus behind fdxA promotor |
The present study |
|
C. jejuni 81116 ΔtsdA/*tsdA NG |
Kanr, Cmr; ΔtsdA mutant complented with tsdA N254G in cj0046 pseudogene locus behind fdxA promotor |
The present study |
|
C. jejuni 81116 ΔtsdA/*tsdA KG |
Kanr, Cmr; ΔtsdA mutant complented with tsdA K252G in cj0046 pseudogene locus behind fdxA promotor |
The present study |
| Plasmids |
|
|
| pEC86 |
Cmr, product from pEC66 and pACYC184 with E. coli ccmABCDEFGH genes |
[20] |
| pC46-fdxA |
C. jejuni complementation vector for integration at the cj0046c pseudogene locus, expressing cloned genes from the fdxA promoter |
[39] |
| pET-N-Strep-TsdACj |
Apr; N-terminal Strep-tag, f1 origin, T7 Promoter, NcoI-HindIII fragment of PCR amplified tsdA in digested pETStrepDsrJ |
[1] |
| pET-N-Strep-TsdACj-C138G |
Apr; C138G mutation introduced into pET-N-Strep-TsdACj |
The present study |
| pET-N-Strep-TsdACj-C138H |
Apr; C138H mutation introduced into pET-N-Strep-TsdACj |
The present study |
| pET-N-Strep-TsdACj-C138M |
Apr; C138M mutation introduced into pET-N-Strep-TsdACj |
The present study |
| pET-N-Strep-TsdACj-M255G |
Apr; M255G mutation introduced into pET-N-Strep-TsdACj |
The present study |
| pET-N-Strep-TsdACj-N254G |
Apr; N254G mutation introduced into pET-N-Strep-TsdACj |
The present study |
| pET-N-Strep-TsdACj-N254K |
Apr; N254K mutation introduced into pET-N-Strep-TsdACj |
The present study |
| pET-N-Strep-TsdACj-K252G |
Apr; K252G mutation introduced into pET-N-Strep-TsdACj |
The present study |
| pET-N-Strep-TsdACj-KMG |
Apr; K252G mutation introduced into pET-N-Strep-TsdACj-M255G |
The present study |
| pET-N-Strep-TsdACj-NMG |
Apr; N254G mutation introduced into pET-N-Strep-TsdACj-M255G |
The present study |
| pET-N-Strep-TsdACj-KNMG |
Apr; K252G andN254G mutation introduced into pET-N-Strep-TsdACj-M255G |
The present study |
| pC46-fdxA-0815HIS |
Cmr; c8j_0815 cloned into pC46-fdxA to produce C-terminal His-tagged TsdA in C. jejuni
|
The present study |
| pC46-fdxA-TsdACj-C138G |
Cmr, C138G mutation introduced into pC46-fdxA-0815 |
The present study |
| pC46-fdxA-TsdACj-M255G |
Cmr, M255G mutation introduced into pC46-fdxA-0815 |
The present study |
| pC46-fdxA-TsdACj-N254G |
Cmr, N254G mutation introduced into pC46-fdxA-0815 |
The present study |
| pC46-fdxA-TsdACj-K252G |
Cmr, K252G mutation introduced into pC46-fdxA-0815 |
The present study |
