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. 2016 Dec 8;16:91. doi: 10.1186/s12935-016-0367-5

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Fluorescent microscopy with monodansylcadaverine staining of MCF-7 and MDA-MB-231 cells. i MCF-7 and ii MDA-MB-231 cells treated with a DMSO and b 3MA served as negative controls and displayed non-specific MDC staining. c Tamoxifen was used as a positive control for acidic vacuoles and displayed clear MDC stained vacuoles. d ESE-15-ol treated cells showed increased MDC staining, while e ESE-15-ol treated cells together with 3MA showed less distinctive MDC staining, indicating partial autophagy inhibition (40× magnification)