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. 2016 Dec 9;6:38757. doi: 10.1038/srep38757

Figure 5. TAIII interferes with the TP-mediated Gq signaling pathway.

Figure 5

(a) Platelets were preincubated with increasing concentrations of TAIII (0–10 μmol/L) for 3 min and then stimulated with 2.5 μmol/L U46619. Calcium concentration was measured by absorbance fluorometry using fura-2-AM. The results are presented as the mean ± SEM (n = 3). Rat PRP was preincubated with TAIII (7.5 μmol/L), stimulated with U46619 (2.5 μmol/L), and immediately treated with or without 20 nmol/L PMA (b) or 400 μmol/L m-3M3FBS (d). Typical real-time ATP secretion and platelet aggregation traces are representative of three independent experiments. Quantitative evaluation of the data from three independent experiments is shown in (c) and (e), and the results are presented as the mean ± SEM. Statistical significance was determined by Student’s t-test. *p < 0.05, compared with U46619-treated platelets.