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. 2016 Dec 9;6:38121. doi: 10.1038/srep38121

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Wild-type and yap1 (a,d,g,j) sod1 (b,e,h,k), trx1/trx2 (c,f,i,l) yeast mutants, transformed with pYES2 and pYES2-CaTLP1, were cultured in SD-URA media overnight and diluted in fresh media (approximate OD₆₀₀ value of 0.01) before being subjected to (a–c) 5 mM H₂O₂, (d–f) 1.2 M NaCl, (g–i) 0.1 M LiCl and (j-l) 0.5 mM menadione treatments. The cultures were incubated at 30 °C and OD₆₀₀ readings were taken at every 1 h. Data shown are the means ± SD of three biological replicates.