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. 2016 Dec 9;6:38121. doi: 10.1038/srep38121

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Copyright © 2016, The Author(s)

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(a) Four-week-old attlp2, wild-type and transgenic plants (OEC-3, OEC-6 and OE-11) were uprooted and subjected to dehydration stress. Scanning electron micrographs display the changes of stomatal movement in transgenics with respect to mutant and wild-type plants. The bar indicates the extent of resolution. (b) Percentage reduction in number of opened stomata (aperture ≥2 μm). (c) Reduction in the stomatal aperture in response to dehydration stress. (d) Histogram shows the percent reduction in stomatal aperture for the indicated lines. The results are the means ± SE for 120 stomata. Transcript abundance of stress-responsive genes viz., RD20 (e), RAB18 (f), bZIP20 (g), polyubiquitin (h) and GroEL (i) were quantified. Quantitative real time PCR was performed using gene-specific primers (Supplemental Table 5). The relative gene expression was evaluated using comparative cycle threshold method with EF-1α as the reference gene. Data shown are the means ± SD of three biological replicates.