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. 2016 Dec 9;6:38121. doi: 10.1038/srep38121

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Copyright © 2016, The Author(s)

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(a) CBB-stained SDS-PAGE gel showing protein bands immunoprecipitated from transgenic Arabidopsis (OE-11) and chickpea, probed with anti-FLAG and anti-CaTLP1 antibodies, respectively. (b) Western blot analysis of immunoprecipitated proteins with anti-Rubisco antibody. (c,d) Confirmation of immunoprecipitates using anti-FLAG and anti-CaTLP1 antibodies. C_C, crude protein from chickpea; C_A, crude protein from Arabidopsis; P, purified protein; P_A, purified protein from Arabidopsis; P_C, purified protein from chickpea; W, wash fraction; P_WT, purified protein from wild-type plants. Arrows indicate band of purified CaTLP1. (e) Surface view interaction of F-Box domain (purple) of CaTLP1 and CaTLP2 (green) with casein kinase 1-like protein 2 (AtCK2) and CaCK1 (cyan) visualized with PyMOL. (f) Interactions of CaTLP1 and CaTLP2 with kinase family proteins showing common as well as exclusive members in the association network. (g) AtTLP2 interaction showing the common casein kinase family protein CK (pink circle) in the network. (h) BiFC assay showing interaction of nYFP-CaTLP1 and cYFP-CaCK1.