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. 2016 Dec 9;6:38622. doi: 10.1038/srep38622

Figure 4. The suppression of inflammasome activation by CAPE is dependent on ASC.

Figure 4

(A–C) 293T cells were transiently transfected with the iGLuc luciferase reporter plasmid (100 ng) and expression plasmids. Luminescence derived from iGLuc activation in each sample was normalized by β-galactosidase activity transfected as an internal control in each sample. (A) *Significantly different from NLRP3 + ASC + caspase-1, 0.5: p = 0.0064, 1–10: p =< 0.0001. (B) *Significantly different from ASC + caspase-1, 0.5: p = 0.0036, 1: p = 0.0001, 5–10: p =< 0.0001. (D) In vitro assay for caspase-1 enzyme activity was performed using a fluorometric caspase-1 assay kit with recombinant human caspase-1 (rCaspase-1; Bio-vision) in the presence or absence of CAPE or Z-VAD-FMK according to the manufacture’s instruction. The fluorescence was recorded at 400 nm after excitation at 505 nm with SpectraMaxM5 (Molecular Devices, Sunnyvale, CA). *Significantly different from rCaspase-1 alone, p = 0.0007. The values represent the means ± SEM (n = 3).