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. 2016 Dec 9;6:38900. doi: 10.1038/srep38900

Figure 1. Schematic diagram of the experimental design used in this study.

Figure 1

Reproductive females were obtained by raising larvae at 25 °C under a 12:12 h light:dark (LD) photoperiod and diapause-destined females by raising larvae at 25 °C under 16:8 LD photoperiod. Reproductive and diapause-destined female adults store energy obtained from plant foods in the ovary and the fat body, respectively, during the 4 day post-eclosion (PE) period. Gut microbiota were collected for sequencing from reproductive (R) and diapause-destined (D) females at 1 and 3 days PE. R-1d, reproductive adult at 1 day PE; R-3d, reproductive adult at 3 days PE; D-1d, diapause-destined adult at 1 day PE; D-3d, diapause-destined adult at 3 days PE.