Table 1. Rate constants for EcFrmR DNA interactions.

Reactiona	kf (M−1 s−1)b	kr (s−1)a	Kd (nM)	kapp (M−1 s−1)
EcFrmR + Pfrm ↔ Pfrm − EcFrmR	13000 ± 390	0.0028 ± 0.000086	220	—
EcFrmR − (Zn)4 + Pfrm ↔ Pfrm − EcFrmR − (Zn)4	5660 ± 165	0.003 ± 0.000078	520	—
Pfrm − EcFrmR + HCHO → EcFrmR − HCHO + Pfrm	—	—	—	4.2
Pfrm − EcFrmR − (Zn)4 + HCHO → EcFrmR − (Zn)4 − HCHO + Pfrm	—	—	—	0.7
EcFrmR + PydhY ↔ PydhY − EcFrmR	1950 ± 1000	0.007 ± 0.00017	3600	—

^aThe BLItz data used to calculate the kinetic parameters shown are provided in Table S4. The data were fitted to a 1:1 binding model to derive k_f, k_r and K_d values using all the sample data simultaneously (Global fitting).

^bValue and standard error.