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. 2016 Dec 9;6:38849. doi: 10.1038/srep38849

Figure 1. STRAP upregulates TLR-mediated pro-inflammatory cytokine production.

Figure 1

(a–c) Overexpression of STRAP enhances IL-6, TNF-α, and IL-1β mRNA, whereas STRAP deficiency diminished those mRNA levels in LPS-treated RAW 264.7 macrophages. Cells stably expressing shRNA-GFP (control), shRNA-STRAP (STRAP k/d), vector (Mock), or GFP-STRAP (GFP-STRAP) were treated with LPS (80 ng/ml) for 12 h. The IL-6, TNF-α, or IL-1β mRNA levels were analyzed by RT-PCR. Expression was quantified by densitometry of bands and reported relative to GAPDH. *P < 0.01 (Student’s t-test). (d–g) IL-6 and TNF-α production is affected by STRAP expression levels in LPS- or Pam3CSK4-stimulated RAW macrophages. Cells were treated with LPS (80 ng/ml) or Pam3CSK4 (100 ng/ml) for 12 h, respectively. IL-6 and TNF-α levels were analyzed by ELISA. *P < 0.01 and **P < 0.005 (Student’s t-test). Data are representative of three independent experiments and are presented as mean ± s.d. in (a–g).