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. 2016 Dec 9;7:1793. doi: 10.3389/fpls.2016.01793

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Copyright © 2016 Leida, Dal Rì, Dalla Costa, Gómez, Pompili, Sonego, Engelen, Masuero, Ríos and Moser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VviERF045 expression pattern and protein sequence comparative analysis. Left panel: real time RT-qPCR analysis of VviERF045 expression profile in different tissues (A): B, Bud; S, Shoot; ML, Mature Leaf; YL, Young Leaf; F, Flower; R, Root; T, Tendril; 5WAA, berry at 5 weeks after anthesis (WAA); 10 WAA, berry at 10 WAA. ML and YL were used as reference samples. (B) VviERF045 expression in different fruit parts: pulp, skin and seed at 10 WAA. Pulp is taken as calibrator (C) VviERF045 expression at different developmental stages, berry developmental stages are indicated as WAA, (v) indicates véraison. (D) VviERF045 expression after 1-MCP and ethephon treatment: points represent 1-MCP treatment, points and lines represent ethephon treatment and continuous line represents control. Error bars represent SD and are based on three biological and two technical replicates. Data were normalized using ubiquitin and tubulin as reference genes. Different letters in the figure mean significant difference (p < 0.05) according to Tuckey’s post hoc test. Right panel: (E) Phylogenetic tree of the ERF amino acid sequences from Prunus salicina [PsERF1a (FJ026009), PsERF1b (FJ026008), PsERF12 (FJ026003), PsERF3a (FJ026005), PsERF3b (FJ026004), PsERF2a (FJ026007), PsERF2b (FJ026006)], Arabidopsis thaliana [AT5G25190 (NP_197901.1), AT1G15360-SHN1 (NP_172988.1), AT5G25390-SHN3 (NP_197921.1), AT5G11190-SHN2 (NP_196680.1)], Solanum lycopersicum [SlSHN1 (XP_004235965.1), SlSHN3 (XP_004240977.1), SlERF1 (NP_001234848.1)] and Vitis vinifera ERF from clade V [VviERF045, VviERF042, VviERF051, VviERF044, VviERF043, VviERF048, VviERF049, VviERF041, VviERF050, VviERF047, VviERF046]. The aa sequences were selected based on these criteria: (i) Grapevine ERFs belonging to the same clade of VviERF045, (ii) Prunus salicina ERF sequences related to fruit ripening, (iii) Best blastx matches to VviERF045 from A. thaliana and S. lycopersicum. Numbers on the branch represent the percentage for bootstrap value n = 1000. (F) Alignment of the amino acid sequences of clade V of the phylogenetic tree. ‘AP2,’‘mm’ and ‘cm’ conserved domains are represented as blue rectangles.