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. 2016 Dec 9;7:1793. doi: 10.3389/fpls.2016.01793

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Copyright © 2016 Leida, Dal Rì, Dalla Costa, Gómez, Pompili, Sonego, Engelen, Masuero, Ríos and Moser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Microscopic analysis of VviERF045 overexpressing lines. (A) Differences in lipids vesicle content in trangenic L6 and WT observed at 50X magnification and by SudanIV staining. LB, lipid bodies. (B) Changes in wax load and cuticle surface morphology of the transgenic lines detected by Scanning Electron Microscopy after freeze fracture (CryoSEM). Upper part: images of transversal section of the adaxial leaf surface. WT and L19 show wax deposition. (2000X) CR, Cuticular Ridge. Lower part: images of the adaxial leaf surface (1000X). WA, Wax Aggregates.