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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2016 Aug 12;165(Pt B):342–355. doi: 10.1016/j.jsbmb.2016.08.001

Fig. 3.

Fig. 3

Selective reaction ion-chromatograms of hydroxy-androgen picolinates and four [13C3]-hydroxy-androgen picolinate internal standards. The sample is from a dilution of CD-FBS serum (0.2 mL) containing 12.5 pg of standard mixture and 100 pg of IS mixture. On-column injection is from: 2.5 pg of hydroxy-androgen picolinate and 20 pg of [13C3]-hydroxy-androgen picolinate prepared by extracting the unconjugated hydroxy-androgen from the matrix. 3α-androstanediol: 5α-androstane-3α, 17β-diol; 3β-androstanediol: 5α-androstane-3β, 17β-diol; 5-androstenediol: 5-androstene-3β, 17β-diol; DHEA: dehydroepiandrosterone; DHT: 5α-dihydrotestosterone; EpiT: epitestosterone; T: testosterone.