Table 1.
Inter- and intra-assay accuracy and precision from determination of nine hydroxy-androgen picolinates.
| T | EpiT | DHEA | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Theoretical (pg) | 1.0 | 50 | 500 | 2.5 | 50 | 500 | 2.5 | 50 | 500 |
| Inter-assay | |||||||||
| Determined(± SD, pg) | 1.11 ± 0.06 | 50.5 ± 0.7 | 497 ± 7 | 2.40 ± 0.07 | 52.6 ± 1.5 | 505 ± 6 | 2.47 ± 0.18 | 51.9 ± 2.8 | 503 ± 3 |
| Accuracy (%) | 111 | 101 | 99.5 | 96.0 | 105 | 101 | 98.8 | 104 | 101 |
| Precision (%) | 5.4 | 1.4 | 1.4 | 2.9 | 2.8 | 1.2 | 7.3 | 5.4 | 0.6 |
| Inter-assay | |||||||||
| Determined(± SD, pg) | 1.07 ± 0.08 | 51.1 ± 0.9 | 502 ± 7 | 2.46 ± 0.14 | 52.0 ± 1.6 | 498 ± 10 | 2.36 ± 0.19 | 50.8 ± 1.6 | 505 ± 11 |
| Accuracy (%) | 107 | 102 | 10 0 | 106 | 104 | 99.6 | 94.4 | 102 | 101 |
| Precision (%) | 7.5 | 1.8 | 1.4 | 5.7 | 3.1 | 2.0 | 8.1 | 3.1 | 2.2 |
| DHT | Androsterone | Epiandrosterone | |||||||
| Theoretical (pg) | 2.5 | 50 | 500 | 2.5 | 50 | 500 | 2.5 | 50 | 500 |
| Inter-assay | |||||||||
| Determined(± SD, pg) | 2.24 ± 0.23 | 50.4 ± 0.7 | 498 ± 2 | 2.40 ± 0.30 | 49.4 ± 1.4 | 506 ± 17 | 2.63 ± 0.45 | 52.1 ± 2.6 | 503 ± 8 |
| Accuracy (%) | 89.6 | 101 | 99.6 | 96.0 | 98.8 | 101 | 105 | 104 | 101 |
| Precision (%) | 10 | 1.4 | 0.2 | 12.5 | 2.8 | 3.4 | 17 | 5.0 | 1.6 |
| Inter-assay | |||||||||
| Determined(± SD, pg) | 2.10 ± 0.09 | 50.8 ± 0.6 | 497 ± 2 | 2.56 ± 0.29 | 49.9 ± 0.6 | 497 ± 7 | 2.95 ± 0.18 | 50.8 ± 0.7 | 494 ± 6 |
| Accuracy (%) | 84.0 | 102 | 99.4 | 102 | 99.8 | 99.4 | 118 | 102 | 98.8 |
| Precision (%) | 4.3 | 1.2 | 0.4 | 11 | 1.2 | 1.4 | 6.1 | 1.4 | 1.2 |
| 3α–androstanediol | 3β–androstanediol | 5-androstenediol | |||||||
| Theoretical (pg) | 1.0 | 50 | 500 | 2.5 | 50 | 500 | 1.0 | 50 | 500 |
| Inter-assay | |||||||||
| Determined(± SD, pg) | 1.02 ± 0.16 | 50.0 ± 2.2 | 506 ± 7 | 2.52 ± 0.26 | 50.3 ± 0.9 | 510 ± 4 | 1.07 ± 0.08 | 50.5 ± 2.3 | 505 ± 9 |
| Accuracy (%) | 102 | 100 | 101 | 101 | 101 | 102 | 107 | 101 | 101 |
| Precision (%) | 15.7 | 4.4 | 1.4 | 10.3 | 1.4 | 0.8 | 7.5 | 4.6 | 1.8 |
| Inter-assay | |||||||||
| Determined(± SD, pg) | 0.96 ± 0.03 | 52.6 ± 1.1 | 504 ± 7 | 2.32 ± 0.10 | 49.4 ± 1.1 | 506 ± 3 | 1.01 ± 0.03 | 51.8 ± 0.3 | 501 ± 7 |
| Accuracy (%) | 96.0 | 105 | 101 | 92.8 | 98.8 | 101 | 101 | 104 | 100 |
| Precision (%) | 3.1 | 2.1 | 1.4 | 4.3 | 2.2 | 0.6 | 3.0 | 0.6 | 1.4 |
All data are presented as a mean with standard deviation in pg on column and obtained using matrix (CD-FBS) matched standards. Precision is given as the percentage of coefficient of variation. SD: standard deviation. LLOQ is highlighted in bold (1.0 pg on column: 2.5 ng ng/dL; 2.5 pg on column: 6.25 ng/dL).T: testosterone; EpiT: epitestosterone; DHEA: dehydroepiandrosterone; DHT: 5α-dihydrotestosterone; 3α-androstanediol: 5-androstane-3α, 17β-diol; 3β-androstanediol: 5-androstane-3β, 17β-diol; 5-androstenediol: 5-androstene-3β, 17β-diol.