Table 2.
Quantitation of hydroxy-androgens and the corresponding conjugates in serum from male and female.
| Type | Hydroxy-androgen (± SD, ng/dL), in triplicate from pooled serum (200 µL each) |
|||||
|---|---|---|---|---|---|---|
| Male |
Female |
|||||
| Unconjugated | Sulfate (−S) | Glucuronide(−G) | Unconjugated | Sulfate (−S) | Glucuronide(−G) | |
| T | 450 ± 16 | ND | 123 ± 6 | 33.0 ± 2.6 | ND | 36.7 ± 1.1 |
| EpiT | ND | ND | ND | ND | ND | ND |
| DHEA | 118 ± 4 | 119507 ± 1823 | 31.1 ± 1.3 | 97.8 ± 0.8 | 122799 ± 602 | 43.9 ± 0.4 |
| DHT | 35.2 ± 2.6 | ND | ND | 10.8 ± 0.3 | ND | ND |
| Androsterone | 20.7 ± 1.0 | 724 ± 58 | 4868 ± 66 | 14.5 ± 0.8 | ND | 4176 ± 32 |
| Epiandrosterone | 9.1 ± 0.6 | 3886 ± 117 | 3591 ± 63 | 18.5 ± 0.9 | 4578 ± 122 | 3679 ± 17 |
| 5-androstenediol | 50.0 ± 4.7 | 6606 ± 391 | NQ | 43.7 ± 2.1 | 7982 ± 780 | NQ |
| 3α-androstanediol | 6.9 ± 0.7 | ND | 520 ± 35 | NQ | ND | 242 ± 4 |
| 3β-androstanediol | NQ | ND | ND | NQ | ND | ND |
ND: not detected. Limit of detection (LOD, S/N≥3) of T, 5-androstenediol and 3α-androstanediol: ~0.4 pg on column (or 1.0 ng/dL); LOD of EpiT, DHEA, DHT, androsterone, epiandrosterone and 3β-androstanediol: ~1.0 pg on column (or 2.5 ng/dL). NQ: not quantifiable (below LLOQ). SD: standard deviation. Triplicate represents three independent determinations.