Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 5;215(5):719–734. doi: 10.1083/jcb.201605046

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Shen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — Astrocytic MyD88 deletion in vivo attenuates the effects of LPS on PTZ-induced seizure susceptibility and excitatory synaptic transmission in juvenile mice. (A) Schematics of experimental procedures of piggyBac (PB)–IUE in MyD88^flox/flox mice (left) and representative image of hippocampal slices from PB-IUE mice (right). Bar, 200 µm. (B) Representative GFP-positive cells electroporated with the PB (left) and control (Ctrl; top right) plasmids in the hippocampus immunohistostained with s100β (top left) and MyD88 (bottom left and top right) and quantification of the percentage of MyD88 expression in GFP-positive astrocytes (bottom right). n = 7 for each group. Bars, 10 µm. Partially deleting astrocytic MyD88 abolished LPS-induced reduction in onset (C) and prolongation of duration (D) of PTZ-induced seizures in young mice. n = 6, 5, 6, and 7 for Ctrl-saline, Ctrl-LPS, PB-saline, and PB-LPS groups, respectively. (E) Representative traces of mEPSC recordings in CA1 pyramidal neurons from P14 IUE mice. Astrocytic MyD88 deletion prevented LPS-induced increases in mEPSC frequency (F) and amplitude (G) in CA1 pyramidal neurons from P14 IUE mice. n = 6, 7, 9, and 9 for Ctrl-saline, Ctrl-LPS, PB-saline, and PB-LPS groups, respectively. *, P < 0.05; **, P < 0.01; ***, P < 0.001; #, P < 0.05; ##, P < 0.01 (in B, t test; in C, D, F, and G, two-way ANOVA with the post hoc Holm-Sidak test). Data are mean ± SEM.