Table 1.
Conditions of enzyme assays.
| Enzyme | Conc. (mM) | Substrate | Buffer | pH | Incubation time (min) | Solubilisate volume (μl) |
| α-Mannosidase | 2.5 | 4-methylumbelliferyl-α-D-mannopyranoside | Phosphate/citrate (0.1 M) | 4.0 | 120 | 40 |
| Hexosaminidase | 5.0 | 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide | Phosphate/citrate (0.1 M) | 4.5 | 30 | 5 10 (PC) |
| Glucuronidase | 2.5 | 4-methylumbelliferyl-β-D-glucuronide | Acetate (0.1 M) | 4.5 | 120 | 20 5 (LSEC) |
| Acid phosphatase | 1.0 | 4-methylumbelliferyl-phosphate | Acetate (0.1 M) | 4.5 | 30 | 10 |
| Aryl sulphatase | 10.0 | 4-methylumbelliferyl-sulfate | Acetate (0.5 M) | 5.5 | 120 | 40 |
| Acid lipase | 0.3 | 4-methylumbelliferyl-oleate | Acetate (0.1 M)* | 4.0 | 60 | 20 |
*Acetate (0.1 M) + 0.1% Triton X-100 + phosphatidylcholine (100 μM) + taurodeoxycholic acid (300 μM).