Table 1.
Protease Profiles Expressed by Several DC Populations
| Protease | XS52 DC | Splenic DC | Bone Marrow DC | BW5147 Thymoma Cells |
| Cathepsin B1 | 133 ± 392 | 123 ± 3.3 | 121 ± 3.3 | 1.5 ± 0.08 |
| Cathepsin C | 34 ± 7 | 16 ± 4 | 0.4 ± 0.1 | <0.01 |
| Cathepsin D/E | 34 ± 6 | 30 ± 14 | 22 ± 2 | 1.6 ± 0.1 |
| Cathepsin H | 2.8 ± 0.8 | 3.5 ± 0.7 | 1 ± 0.2 | 0.9 ± 0.2 |
| Cathepsin J | 26 ± 0.3 | 0.7 ± 0.3 | 3.0 ± 0.1 | 0.2 ± 0.09 |
| Cathepsin L | 14 ± 0.6 | 26 ± 11 | 19 ± 0.6 | 0.6 ± 0.08 |
| BLT esterase | 25,000 ± 400 | 58,000 ± 4,000 | 21,300 ± 100 | <100 |
| Suc-FLF-SBz esterase | 1,900,000 ± 61,000 | 310,000 ± 10,800 | 1,150,000 ± 10,200 | 12,000 ± 100 |
| Suc-AAPFSBZ esterase | 433,000 ± 2,900 | 134,000 ± 7,300 | 360,000 ± 6,100 | 3,400 ± 600 |
1Extracts prepared from the indicated cell types were examined for protease activities using a panel of standard substrates. 2Enzymatic activities are expressed as nmol/min/mg soluble protein. Data shown represent the mean ± SD from three independent preparations.