TABLE 1.

PCR oligodeoxynucleotide primers used in this study

Primer name	Sequencea	Primer positionb	PCR product size (bp)
23S-F	5′-TCATAGTCTCCTGGCTATCC-3′	2103	175
23S-R	5′-GACCTGCACGAATGGTGTAA-3′	1928
23Sb-F	5′-ATTCCGGTCCTCTCGTACTA-3′	2642	212
23Sb-R	5′-CGCCACCAAGAGTTCATATC-3′	2431
L4-F	5′-CGCTCAACAAGTTCCTGCAAAG-3′	26	434
L4-R	5′-AATGCCCGTCAGGGATGTTTAG-3′	459
L22-F	5′-CTTTCTGACCCATGATCC-3′	−28	405
L22-R	5′-GCCATCCTGTGAAGAAAG-3′	386
Erm-1	5′-GA(A/G)ATIGGIIIIGGIAA(A/G)GGICA-3′		530
Erm-2	5′-AA(C/T)TG(A/G)TT(C/T)TTIGT(A/G)AA-3′
KsgA-F	5′-ATTACGCAAGCGCTACGGCTAT-3′	−93	930
KsgA-R	5′-CCTTTAACCCGCTTGCATCT-3′	833 + 3
YgeD5-F	5′-GTGGTCTGACAAGACATGTA-3′	−181	1,193
YgeD5-R	5′-CATGGAGAGGCACTTGATA-3′	1011
YgeD3-F	5′-TTTGCTTGTTCGATACTATT-3′	940	819
YgeD3-R	5′-TAAGAACGATTTACGGTATCTTA-3′	1698 + 60
Yjjk-3	5′-GCTCCTCGTGTTAGATAC-3′	1593 + 19	939
Yjjk-3int	5′-ACGACACGATCATTATCTAC-3′	673
Yjjk-5	5′-GGTCTATACGCAAGTCTC-3′	−121	957
Yjjk-5int	5′-ACTTGACTCGCTCGTGAT-3′	835

^aT, thymidine; C, cytosine; A, adenosine; G, guanine; I, inosine; (X/Y), nucleotides X and Y present in equal parts.

^bBase position relative to the translational start site of the gene cited in the primer name; a plus sign indicates base pairs situated after the 3′ end of the gene.