Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 5;12:3083–3092. doi: 10.2147/NDT.S121183

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Yao et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

miR-455 upregulation restrained neuronal cell death and weakened ischemic brain infarction.

Notes: (A) Neurons were treated with miR-455 mimics or miR-455 inhibitor, and miR-455 expression levels were detected by qRT-PCR, F=7.17. (B) The CCK-8 assay was used to detect cell survival, and the neuronal cell death rate was increased after OGD for 24 hours, t=4.49. (C) The neuronal cell treated with miR-455 mimics showed a decreased death rate after OGD for 24 hours, t=3.38. (D) The miR-455 inhibitor significantly increased death rate in neurons after OGD for 24 hours, t=2.53. (E) miR-455 level was increased in brain treated with miR-455 mimics after MCAO for 24 hours, n=5, t=4.51. (F) The level of miR-455 was reduced in brains treated with miR-455 inhibitor after MCAO for 24 hours, n=5, t=4.57. (G) The infarction volume of ischemic brain treated with miR-455 mimics was reduced, n=5, t=2.45. (H) The infarction volume of ischemic brain treated with miR-455 inhibitor was aggravated, n=5, t=3.47. *P<0.05.

Abbreviations: qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, Cell Counting Kit-8; OGD, oxygen–glucose deprivation; MCAO, middle cerebral artery occlusion.

miR-455 upregulation restrained neuronal cell death and weakened ischemic brain infarction.

Notes: (A) Neurons were treated with miR-455 mimics or miR-455 inhibitor, and miR-455 expression levels were detected by qRT-PCR, F=7.17. (B) The CCK-8 assay was used to detect cell survival, and the neuronal cell death rate was increased after OGD for 24 hours, t=4.49. (C) The neuronal cell treated with miR-455 mimics showed a decreased death rate after OGD for 24 hours, t=3.38. (D) The miR-455 inhibitor significantly increased death rate in neurons after OGD for 24 hours, t=2.53. (E) miR-455 level was increased in brain treated with miR-455 mimics after MCAO for 24 hours, n=5, t=4.51. (F) The level of miR-455 was reduced in brains treated with miR-455 inhibitor after MCAO for 24 hours, n=5, t=4.57. (G) The infarction volume of ischemic brain treated with miR-455 mimics was reduced, n=5, t=2.45. (H) The infarction volume of ischemic brain treated with miR-455 inhibitor was aggravated, n=5, t=3.47. *P<0.05.

Abbreviations: qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, Cell Counting Kit-8; OGD, oxygen–glucose deprivation; MCAO, middle cerebral artery occlusion.