TABLE 3.
Time-dependent hyphal damage measurement by XTT viability staining for four isolatesa
| Isolate | Time (h) | % XTT activityb
|
||
|---|---|---|---|---|
| SMX ×1 + CAS ×1 | SMX ×2 | CAS ×2 | ||
| A. niger 6 | 18 | 3 ± 2c | 27 ± 1 | 14 ± 4 |
| 24 | 2 ± 2c | 61 ± 7 | 11 ± 4 | |
| 30 | 82 ± 4 | 89 ± 11 | 100 ± 4 | |
| A. flavus 1 | 18 | 46 ± 2c | 86 ± 8 | 57 ± 2 |
| 24 | 18 ± 3c | 33 ± 8 | 28 ± 2 | |
| 30 | 14 ± 4c | 55 ± 7 | 26 ± 3 | |
| A. fumigatus 7 | 18 | 8 ± 1c | 26 ± 7 | 18 ± 1 |
| 24 | 16 ± 1 | 46 ± 7 | 20 ± 1 | |
| 30 | 24 ± 6c | 72 ± 12 | 45 ± 4 | |
| A. terreus 5 | 18 | 0 ± 3c | 27 ± 3 | 22 ± 5 |
| 24 | 0 ± 0c | 48 ± 8 | 12 ± 2 | |
| 30 | 2 ± 0c | 22 ± 3 | 10 ± 0 | |
a
Data for all time points are normalized to the optical density readings for internal control wells containing the strain grown in the absence of drug(s). Values are means ± standard deviations of results for four wells.
b
×1, the MEC; ×2, two times the MEC.
c
P < 0.05 compared to the results for both SMX and CAS at two times the MEC.