Table 2.
Sequences of miRNA mimics and PCR primers.
| NAME | SEQUENCE miRNA MIMICS— |
|---|---|
| miR-K1 | AUUACAGGAAACUGGGUGUAAGC (Sense) |
| UUACACCCAGUUUCCUGUAACUU (Antisense) | |
| Primers for constructing 3′UTR WT and mutant reporters | |
| RAB23 Site 1 WT |
AGTGGTACCTGCAAAATGAGCTTGGGTTT (Forward) |
| AGTCTCGAGTGTGGGACTGACAGCTCTTG (Reverse) | |
| RAB23 Site 1 mutant |
AGTCATTCAGGAGGTGGACAGTAGTGTGGTGATGC (Forward) |
| GCATCACCACACTACTGTCCACCTCCTGAATGACT (Reverse) | |
| RAB23 Site 2 WT |
AGTGGTACCCAAGAGCTGTCAGTCCCACA (Forward) |
| AGTCTCGAGATTTTGCCCCCAAAACCTAT (Reverse) | |
| RAB23 Site 2 mutant |
AGCATTGCAAAATGACATTAAATAACTTTTATT (Forward) |
| AATAAAAGTTATTTAATGTCATTTTGCAATGCT (Reverse) | |
| hnRNPU WT | AGTGGTACCAGGACGAGGAAACAATCGTG (Forward) |
| AGTCTCGAGAGCTTACCCCCTCCACTACC (Reverse) | |
| hnRNPU Mutant | GGTTCTACATTTTATGACATTAATGT-GACTTTTT (Forward) |
| AAAAAGTCACATTAATGTCATAAAATGTAGAACC (Reverse) |