TABLE 2.
Primer sequences
| Name | Sequencea | Annealing site | Restriction enzyme |
|---|---|---|---|
| GFPf | 5′-GGG ATCGAT ATGAGTAAAGGA-3′ | +1 (GFP) | ClaI |
| URA3r | 5′-AAA CTGCAG AAGGACCACCTTT-3′ | +924 (URA3) | PstI |
| 5′CDR4f | 5′-CCC GGTACC GGCTAGCAGTTTGAG-3′ | −325 (CDR4) | KpnI |
| 5′CDR4r | 5′-GG CTCGAG CGCATCTGCTGCAGG-3′ | +1764 (CDR4) | XhoI |
| 3′CDR4f | 5′-CACCTGCAGGGGTGCAATGCA-3′ | +2366 (CDR4) | None |
| 3′CDR4r | 5′-CCCG AGCTC CAACGTTTACGTCT-3′ | +4458 (CDR4) | SacI |
| MDR1pf | 5′-CAA CTCGAG CACACAGCCGTGAATCTTA-3′ | −5421 (CaMDR1) | XhoI |
| MDR1pr | 5′-CCC ATCGAT GTGAAGTTCTATGTAAGTAGATG-3′ | −1 (CaMDR1) | ClaI |
| CDR1pf | 5′-CC CTCGAG GTTACTCAATAAGTATTAA-3′ | −1182 (CDR1) | XhoI |
| CDR1pr | 5′-CC ATCGAT AATTTTTTTCTTTTTGACC-3′ | −1 (CDR1) | ClaI |
| CDR2pf | 5′-CC CTCGAG ACTAGAAGGTTATCAAGA-3′ | −866 (CDR2) | XhoI |
| CDR2pr | 5′-CCC ATCGAT ATGTTTTTATTGTATGTG-3′ | −1 (CDR2) | ClaI |
| ACT1pf | 5′-CC CTCGAG AGAGCTATTAAGATCACCAG-3′ | −1017 (ACT1) | XhoI |
| ACT1pr | 5′-CC ATCGAT TTTGAATGATTATATTTTTTT-3′ | −1 (ACT1) | ClaI |
| E | 5′CCACATCAGGAGATTATTCGAGCT-3′ | −399 (CDR4) | None |
| B | 5′-ACATCACCATCTAATTCAACAAG-3′ | +42 (GFP) | None |
| C′ | 5′-TTTCCTATGAATCCACTATTGAACC-3′ | +208 (URA3) | None |
| F | 5′-TCCCTGATAGCACTTGGCATTTAT-3′ | +4630 (CDR4) | None |
| G | 5′-GGAGTTCTTCGGAAATTGACCATAAAA-3′ | +1847 (CDR4) | None |