TABLE 3.

Effect of FCZ on permeation of C. albicans cells

Populationa	MPC (μg of FCZ/ml)	% Permeated cellsb
Planktonic JK21 cells	128	11
Planktonic Ca30 cellsc	1	95
Planktonic CaI4 cellsc	1	94
Adherent Ca30 cellsc	128	50
Adherent CaI4 cellsc	128	48
24-h Ca30 biofilmsc	128	43
24-h CaI4 biofilmsc	128	39
Detached Ca30 cells	4	91
Detached CaI4 cells	4-8	92
24-h daughter Ca30 cells	2	98
24-h daughter CaI4 cells	2	96
24-h planktonic Ca30 cells	1-2	94
24-h planktonic CaI4 cells	2	96
48-h daughter Ca30 cells	1-2	96
48-h daughter CaI4 cells	2-4	91
48-h planktonic Ca30 cells	1-2	90
48-h planktonic CaI4 cells	4	94

^aThe permeation of C. albicans cells by FCZ was evaluated with planktonic cells, attached cells, mature biofilms, and biofilm daughter cells.

^bPercentage of cells with a mean fluorescence intensity at 620 nm (FL2) of greater than 1,000 at the indicated MPC.

^cUnlike planktonic cells, adherent and biofilm cells were subjected to mild sonication prior to flow cytometric analysis; nonetheless, adherent and biofilm cells showed less than 50% the permeation shown by plantonic cells.