TABLE 6.
Effect of early adherence on gene expression
| Strain | Construct | Mean ± SD relative fluorescence (FL1)a (arbitrary units; logarithmic scale) of the following cells:
|
||
|---|---|---|---|---|
| Planktonic | Adherent | Adherent cell/planktonic cell ratio | ||
| CaI4 | Parental | 47.71 ± 0.62 | 51.89 ± 0.92 | 1 |
| CaCMR5 | CaMDR1 promoter-yEGFP | 98.89 ± 17 | 103.43 ± 11.3 | 0.95 |
| CaCMR4 | ACT1 promoter-yEGFP | 43.34 ± 0.33 | 93.56 ± 6.62 | 2.2 |
| CaCMR7 | CDR1 promoter-yEGFP | 42.29 ± 1.04 | 220.03 ± 3.34 | 5.2 |
| CaCMR8 | CDR2 promoter-yEGFP | 44.63 ± 0.45 | 73.9 ± 3.35 | 1.65 |
a
From three independent experiments. The Student t test reflected P values of 0.005 for CaCMR1, 0.0001 for CDR1, and 0.005 for CDR2, indicating 99% confidence.