Fig.7.

Treatment with tocotrienol-rich fraction (TRF) has no effect on microglial activity. Twenty-micrometer brain sections from wild-type mice (WT; n = 4) and AβPP/PS1 mice supplemented with water (Cont; n = 4), TRF (n = 4), or palm oil stripped of vitamin E (PO; n = 4) were stained with anti-Iba1 antibody for detection of activated microglia. A) Representative images of the hippocampus and cortex from WT, Cont, TRF, and PO groups. B) Control mice show significantly increased in the immunoreactivity in the hippocampus and cortex compared with WT, while immunoreactivity does not differ among Cont, TRF, and PO groups. Data represent mean±S.E.M. Significance (Bonferroni post hoc test after analysis of variance): ^‡p < 0.05, ^‡‡p < 0.01 versus WT mice. Scale bar: 100 μm.