Fig.8.

Tocotrienol-rich fraction (TRF) supplementation does not affect brain levels of Aβ in AβPP/PS1 mice. Hippocampus and cortex from AβPP/PS1 mice supplemented with water (Cont; n = 4), TRF (n = 4), or palm oil stripped of vitamin E (PO; n = 4) were homogenized and soluble (Fig. 8A–E) and insoluble (Fig. 8F–I) Aβ fractions extracted. Levels of Aβ₄₀, Aβ₄₂, and Aβ oligomers were determined by enzyme-linked immunosorbent assay. No significant differences in soluble and insoluble Aβ levels were found among groups, although insoluble Aβ₄₀ and Aβ₄₂ in the cortex was lower in the TRF-supplemented group (Fig. 8G and I). Data represent mean±S.E.M. No significance by Bonferroni post hoc test after analysis of variance.