Fig.7.

Altered localization of HMGB1in aged human tau transgenic mouse cortical tissue compared to wildtype. HMGB1 co-localized with tau oligomers. A-C) HMGB1 (red) was detected primarily co-localizing with the nucleus (arrow) in WT mice with negligible T22 positive tau oligomers (green). D-F) HMGB1 translocated out of the nuclei (arrowhead) in 24-month-old Htau mice, representative of the activation of HMGB1 as a pro-inflammatory cytokine. Enlargement in (D-F) illustrates the translocation of HMGB1 out of the nucleus and co-localization with T22 positive tau oligomers. G) The HMGB1 corrected cell fluorescence showed a significant increase in aged Htau compared to WT. This is consistent with HMGB1 translocating to the cytoplasm during inflammatory signaling. ^*p < 0.05. Scale bar 20 μm.