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. 2004 Sep;48(9):3268–3271. doi: 10.1128/AAC.48.9.3268-3271.2004

FIG. 3.

FIG. 3.

Intracellular content of arsenic (As) in wild-type and car1 mutant cells grown in the presence of Mel Ox, Mel B, and thiamine. The samples were prepared and the intracellular arsenic content was determined as indicated in Materials and Methods. (A) Wild-type cells were cultivated in MM containing Mel Ox at the following concentrations. For lane 1, 0 μM; lane 2, 0.1 μM; lane 3, 0.5 μM; lane 4, 1 μM; lane 5, 5 μM. (B) Cells were cultivated in MM containing 1 μM Mel Ox and the following concentrations of exogenously added thiamine: for lane 1, 0 μM; lane 2, 0.01 μM; lane 3, 0.1 μM; lane 4, 1 μM; lane 5, 10 μM. (C) Cells of the wild type (lanes 1 and 3) and the Δcar1::ura4 mutant (lanes 2 and 4) were cultivated in MM containing 2 μM Mel Ox (lanes 1 and 2) or 20 μM Mel B (lanes 3 and 4).