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. Author manuscript; available in PMC: 2016 Dec 9.
Published in final edited form as: Sci Signal. 2016 Apr 26;9(425):ra43. doi: 10.1126/scisignal.aad0694

Fig. 6. GPR83 functionally interacts with GPR171.

Fig. 6

(A) The effect of expressing mGPR171 on [125I]Tyr-rPEN binding to membranes (30 μg) from CHO hGPR83 cells. (B) The effect of expressing mGPR171 on surface abundance of hGPR83 in CHO hGPR83 cells. (C) The effect of expressing mGPR171 on PEN-mediated GTPγS binding in CHO hGPR83 cells. (D) The effect of shRNA-mediated knockdown of GPR171 on [125I]Tyr-rPEN binding to Neuro2A cells. The GPR171 shRNA has been described previously (13). (E) The effect of shRNA-mediated knockdown of GPR171 in Neuro2A cells on PEN-mediated GTPγS binding. (F) The effect expressing mGPR171 in CHO hGPR83 cells (2 × 105 cells) on 100 nM mPEN-mediated hGPR83 internalization. (G) The effect of expressing hGPR83 in CHO mGPR171cells (2 × 105 cells) on bigLEN (100 nM)–mediated mGPR171 internalization. Data (A to G) represent means ± SE (n = 3 to 6 independent experiments). **P < 0.01; ***P < 0.001 [t test for (B); for details of statistical analysis, see table S1].