FIG. 2.

Activities of empty PC-SA liposomes, free SAG, and PC-SA-encapsulated SAG against L. donovani amastigotes in resident peritoneal macrophages from BALB/c mice. Cells were infected with L. donovani promastigotes and incubated with increasing concentrations of PC-SA (A), SAG (B), and PC-SA-entrapped SAG (C) for 72 h at 37°C. Levels of infection efficiency (percent infected cells [top panels]), intracellular growth (parasites per infected cell [middle panels]), and parasite survival (parasite per 100 cells [bottom panels]) of drug-treated cells are shown. Infected control macrophages contained 8.03 ± 0.117 amastigotes per macrophage. The bars show the standard errors for three replicates and are representative of two independent experiments.