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. 2004 Sep;48(9):3373–3381. doi: 10.1128/AAC.48.9.3373-3381.2004

FIG. 1.

FIG. 1.

SSCP analysis of katG315, inhA (promoter and structural ORFs), oxyR-ahpC intergenic region, and three regions of kasA in INH-resistant M. tuberculosis. All PCR products were denatured in the presence of 95% deionized formamide and electrophoresed by using specific conditions shown in Table 2 and by using a GenePhor electrophoresis system (Amersham Biosciences). (A) katG315 (145-bp PCR products). Lanes: 3 and 10, wild-type control (H37Rv); 1, W341S; 2, S315R; 4 and 6, S315N; 7, S315G; 5 and 8, wild type (susceptible isolates); and 9, S315T. (B) inhA promoter (187-bp PCR products). Lanes: 1, wild-type control (H37Rv); 2 and 4, C-17T; 3, C-15T. (C) inhA structural gene (codon 16) (222-bp PCR products). Lanes: 1 and 6, wild-type control (H37Rv); 2, I21V; 3, wild type (susceptible isolate); 4, L44L; 5, I21T. (D) oxyR-ahpC intergenic region (264-bp PCR products). Lanes: 1, 7, and 13, wild-type control (H37Rv); 2, oxyR; 5, C−15T; 6, C−39T; 8, C−10T; 9, G−48A; 10, C−12T; 11, G-9A; 12, C-39T; 3, and 4, wild-type (susceptible isolates). (E) kasA region 3 (269-bp PCR products). Lanes: 1, wild-type control (H37Rv); 2, 4, and 5, H180H; 3, G149G; and 6, wild type (susceptible isolates). (F) kasA region 4 (263-bp PCR products). Lanes: 1, wild-type control (H37Rv); 2 and 3, G269S. (G) kasA region 5 (223-bp PCR products). Lanes: 1, wild-type control (H37Rv); 2, G318G; and 3, wild type (susceptible isolates).