TABLE 1.

Oligonucleotide primers used for PCR-SSCP analyses of katG, inhA (regulatory and structural regions), kasA, and the oxyR-ahpC intergenic region

Accession no.	Primer	Primer sequence (5′ to 3′)	Nucleotide locationa	PCR annealing temp (°C)	PCR product size (bp)
Z97193	katG-315F	AGA GCT CGT ATG GCA CCG GA	32059	58	145
katG-315R	CCA GCA GGG CTC TTC GTC AG	31915
	katG-463F	CTG CTG TGG CAG GAT CCG GT	31636	60	162
katG-463R	GCG CTT GTC GCT ACC ACG GA	31494
V66801	inhA-1F	GCT GAG TCA CAC CGA CAA ACG	909	60	187
inhA-1R	CCA GGA CTG AAC GGG ATA CGA	1075
V02492	inhA-16F	TGA CAC AAC ACA AGG ACG CA	1779	52	222
inhA-16R	GTT TTG CAC GTC GAG TTC GA	1981
AF106077	inhA-94F	GCA AAA CGA GGA GCA CCT GGC	1994	63	182
inhA-94R	AAT ACG CCG AGA TGT GGA TGC	2156
V16243	ahpC-F	CTT GCG GCA CTG CTG AAC CAC	7147	60	264
ahpC-R	ACA GGT CAC CGC CGA TGA GAG	7389
Z70692	kasA-1F	AAC GTT CAG GCG AGG CTT GA	30631	68	262
kasA-1R	ATG TCG AGT CGG CCC ATG TG	30873
	kasA-2F	CGG TCA CCT CAA GGA TCC GG	30844	58	267
kasA-2R	GCA CCG TTG GGC ATG ATC AT	31092
	kasA-3F	GGA AGG TGT CCC CGC TGG CC	31066	63	269
kasA-3R	TCA GGC TCG TCG TTG CGG GT	31314
	kasA-4F	TTC TCC ATG ATG CGG GCC AT	31290	68	263
kasA-4R	AGC TCC AGC GAG CGA GTC AT	31533
	kasA-5F	CCG ATG GTG TTC GTG CCG GT	31507	63	223
kasA-5R	ACC GAC TCG AGC GCA CCG AC	31710
	kasA-6F	GTC TGC GTC GGG CCA CTC GA	31682	63	255
	kasA-6R	CTC GCG GCG ACC CGC GAT GTC	31917

^aNumbers refer to the nucleotide position within the referenced GenBank sequence of the 5′ primer terminus.