TABLE 3.

Oligonucleotide primers used for PCR and the sequencing reaction of katG, inhA (regulatory and structural), the oxyR-ahpC intergenic region, and kasA

Accession no.	Primer	Primer sequence (5′ to 3′)	Nucleotide locationa	PCR annealing temp (°C)	PCR product size (bp)
U41314	BC 48	TGG CCG CGG CGG TCG ACA TT	725	58	322
BC 51R	CCA GCA GGG CTC TTC GTC AG	1027
Z79701	InhA-1	CCT CGC TGC CCA GAA AGG GA	870	60	248
InhA-2	ATC CCC CGG TTT CCT CCG GT	1098
	InhA-5	TGG ACG GCA AAC GGA TTC TGG	1813	60	366
InhA-6	ACG AAT ACG CCG AGA TGT GGA	2158
Z81451	AhpC-1	GCC TGG GTG TTC GTC ACT GGT	7455	60	359
AhpC-2	CGC AAC GTC GAC TGG CTC ATA	7117
Z70692	KasA-1	CGT TCA GGC GAG GCT TGA GGC	93	68	700
KasA-5	CAG GCT CGT CGT TGC GGG TC	773
	KasA-4	TGC CCA TCG CGG CGT TCT CCA	736	68	703
KasA-8	GTC CGA CTC GCC CCC GCA AGC	1418
	KasA-2	CCA GAC CGG TTC GCC GTT GTT	435
	KasA-6	CGC CGG CGG ACA TCG ACC AC	1024
	KasA-7	GCC GTG CCG TGC GCG TTG A	1045
X68081	KatG-1	GCC CGA TAA CAC CAA CTC CTG	1947	68	680
KatG-5	CAG ATC CCG CTA CCG CTG TA	2606
	KatG-2	CGC CGA CTA CGG CCA CTA	2254
	KatG-3	GCC ACG CCA TCC GGA TAA	2238
	KatG-4	CCT GGC TCG GCG ATG A	2586	68	648
KatG-9	CTC GGT GGA TCA GCT TGT ACC	3213
	KatG-6	CTC GAT GGC ACC GGA ACC	2884
	KatG-7	CGT CGG GGT GTT CGT CCA TAC	2936
	KatG-8	GAG GAA TTG GCC GAC GAG TT	3182	68	667
KatG-13	TCT CAG GGG CAC TGA GCG TAA	3828
	KatG-10	CAA GTC GGG TGG GAG GTC AA	3482
	KatG-11	CTC TTC CAG GGT GCG AAT GAC	3527
	KatG-12	GCC GAG TAC ATG CTG CTC GAC	3794	60	452
	KatG-14	CGG CGG GTT GTG GTT GA	4229

^aNumbers refer to the nucleotide position within the referenced GenBank sequence of the 5′ primer terminus. Oligonucleotides KasA-2, KasA-6, KasA-7, KatG-2, KatG-3, KatG-6, KatG-7, KatG-10, and KatG-11 were used only for sequencing reactions.