Fig. 4.

Apoptosis detection. The four quadrants of rectangular gate—Q1, Q2, Q3 and Q4 represents necrotic, late apoptotic, live and early apoptotic cells respectively. a Representative dot plot graph of cells: i Untreated control, ii Positive control—Camptothecin, iii Raw Cumin (250 μg/mL) and iv Spent Cumin (250 μg/mL); b Percentage of cells in early and late apoptotic stage. Each value represents mean ± SD (standard deviation) from triplicate measurements. *ϕ significantly different from control (early stage and late apoptosis respectively; p < 0.05). #ϒ significantly different from Camptothecin (early stage and late stage apoptosis respectively; p < 0.05)