Fig. 2. Detection of mutations associated with clarithromycin resistance in Helicobacter pylori strains by PCR-RFLP. Electrophoresis in a 3% agarose gel of 23S rDNA-PCR-RFLP. Lane 10 is the 100-bp molecular weight marker. Lane 9 is the positive control. Lanes 1, 2, 3, 5, 7 and 8 show the amplifications products of samples containing the A2142G mutation after digestion with the MboII restriction enzyme. Lanes 4 and 6 are the 23S-PCR negative controls.
