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. 2016 Nov 21;12(11):e1006445. doi: 10.1371/journal.pgen.1006445

Fig 7. Circadian rhythms of locomotor activity under DD and DimDim, but not under LL or LD cycles, are affected by blocking the pineal clock.

Fig 7

Analysis of locomotor activity of 6–8 dpf Tg(aanat2:EGFP-ΔCLK) larvae (ΔCLK) and control larvae under various lighting conditions. A–D, left chart: The average distance moved (cm/10 min) is plotted on the y-axis and circadian time (CT) is plotted on the x-axis; error bars stand for SE (n = 24); black, white and diagonally lined bars represent dark, light and dim light, respectively. A–D, right chart: Distribution of the G-factors (see 'Fourier analysis' in S1 Text) of Tg(aanat2:EGFP-ΔCLK) and control larvae; the median G-factor value for each group is indicated (red lines). (A) Circadian rhythms of locomotor activity under DD, after entrainment by 5 LD cycles, are affected by blocking the pineal clock; a significant difference in the distribution of G-factors was found between Tg(aanat2:EGFP-ΔCLK) and control larvae (p<0.0001, Kolmogorov-Smirnov test). (B) Circadian rhythms of locomotor activity under DimDim, after entrainment by 3 LD cycles and 2 light-dim light (LDim) cycles, are affected by blocking the pineal clock; a significant difference in the distribution of the G-factors was found between Tg(aanat2:EGFP-ΔCLK) and control larvae (p<0.0001, Kolmogorov-Smirnov test). (C) Circadian rhythms of locomotor activity under LL, after entrainment by 5 LD cycles, are NOT affected by blocking the pineal clock. (D) Circadian rhythms of locomotor activity under LD cycles are NOT affected by blocking the pineal clock.