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. 2016 Dec 6;14(12):e2000322. doi: 10.1371/journal.pbio.2000322

Fig 3. Responsiveness of the NIL Col-S2 and mpk12 mutants to stomatal opening and closing stimuli.

Fig 3

(A) Stomatal opening induced by 100 ppm CO2 in whole plants (58 min after induction; n = 12–13). (B) Light-induced stomatal opening inhibited by 2.5 μM ABA in whole plants (24 min after induction; n = 16–18). (C) Stomatal closure induced by 800 ppm CO2 in whole plants (10 min after induction; n = 12–13). (D) Stomatal closure induced by spraying whole plants with 5 μM ABA solution (24 min after induction; n = 12–14). (E) MPK12 is required for the bicarbonate (HCO3-)-induced slow type anion channel activation in guard cell protoplasts. Upper panels show typical whole guard cell protoplast recordings with 11.5 mM free HCO3- added to the pipette solution, and lower panels show average steady-state current-voltage relationships for wild-type (Col-0), NIL Col-S2, and mpk12-4 after treatment with mock or 11.5 mM HCO3- (n = 4–8 per line and treatment). Small letters (A, C) and asterisks (B, D) indicate statistically significant differences according to one-way ANOVA and two-way ANOVA with Tukey HSD for unequal sample size post hoc tests (p < 0.05), respectively. Error bars mark ± SEM. The raw data for panels A–E can be found in S1 Data.