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. 2016 Dec 6;14(12):e2000322. doi: 10.1371/journal.pbio.2000322

Fig 5. Regulation of HT1 by MPK12.

Fig 5

(A) Inhibition of HT1 kinase activity in vitro by different versions of MPK12 (MPK12 G53R—Cvi-0 version of MPK12; MPK12 K70R—inactive kinase; MPK12 Y122C—hyperactive kinase). Upper panel: autoradiography of the SDS PAGE gel; lower panel: Coomassie-stained SDS PAGE. Reaction mixture was incubated for 30 min. (B) Casein phosphorylation by HT1 with different MPK12 concentrations (mean ± SEM; n = 3). The raw data can be found in S1 Data. (C) Kinase-dead HT1 K113M was not in vitro phosphorylated by different versions of MPK12, and only MPK12 and MPK12 (Y122C) display clear autophosphorylation activities.