Fig 6.

Effect of siRNA HSP47 transfection on growth (A), viability (B) and silencing of HSP47 mRNA (C) of PSCs. PSCs from normal rat were treated with RNAiMAX, RNAiMAX siRNA HSP47 (10nM) or medium alone and were incubated for 0–3 days at 37°C at 5×10⁴ cells density / 60mm dish for cell counting (A), for measurement of cell viability (B) by dye exclusion assay, and for quantitative measurement of HSP47 mRNA and GAPDH mRNA by qPCR method (C). Note that PSCs treated with siRNA HSP47 showed significant reduction of cell number (A) and cell viability (B) with concomitant suppression of HSP47 mRNA (C) as incubation time prolonged.