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. 2016 Dec 9;11(12):e0166940. doi: 10.1371/journal.pone.0166940

Fig 3. miR-125b negatively regulated SGPL1 in trophoblast cells.

Fig 3

(A) The expression of miR-125b in HTR8/SVneo cells transfected with miR-125b mimics (miR-125b), scramble control (NC) or mock was revealed by Real-time qPCR. (B) The expression of miR-125b in HTR8/SVneo cells transfected with miR-125b inhibitor, scramble inhibitor (NC inhibitor) or mock was revealed by Real-time qPCR. Relative expression of miR-125b was normalized by U6. Both mRNA (C) and protein (E) levels of SGPL1 in HTR8/SVneo cells transfected with scramble control (NC) and miR-125b mimics (miR-125b) were revealed by Real-time qPCR and western blot. Both mRNA (D) and protein (F) levels of SGPL1 in HTR8/SVneo cells transfected with miR-125b inhibitor (miR-125b inhibitor) and scramble control inhibitor (NC inhibitor) were revealed by Real-time qPCR and Western blot. All experiments were repeated ≥3 times independently in identical conditions. Results are presented as mean ± SEM. Statistical comparison in separate groups between miR-210 and NC was performed using SPSS, with p<0.05 considered as significant. *p < 0.05, **p < 0.01.