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. 2016 Dec 9;11(12):e0166940. doi: 10.1371/journal.pone.0166940

Fig 4. miR-125b directly targets 3’-UTR of SGPL1 transcript.

Fig 4

(A) Schematic map of luciferase reporter assay constructs. The miR-125b target site within the 3’-UTR of SGPL1 was shown as black box. Sequences below indicated predicted miR-125b target site on wild-type (pmir-SGPL1) 3’-UTR, its mutated derivative (pmir-SGPL1-M), and the pairing region of miR-125b. (B) Luciferase assay in HTR8/SVneo cells transfected with pmir-SGPL1 and pmir-SGPL1-M reporter together with miR-125b or NC separately. The cells were harvested 48h later for luciferase assays. All experiments were repeated 3 times independently in identical conditions. Results are presented as mean ± SEM. Statistical comparison in separate groups between miR-210 and NC was performed using SPSS, with p<0.05 considered as significant. *p < 0.05, **p < 0.01.