Fig 5. miR-125b enhanced IL-8 production in HTR8/SVneo cells.
The human trophoblast cells, HTR/SVneo, were planted in 24-well plates (5×105 cells/ml per well) (Corning, Tokyo, Japan). The culture supernatants were collected, filtered, and stored at –80°C until titration of IL-8. The expression of IL-8 in HTR8/SVneo cells transfected with miR-125b mimics, miR-125b inhibitor and SGPL1 siRNA (si-SGPL1) and their corresponding controls were tested using Real-time qPCR (A) and Western (B). The concentrations of IL-8 in the supernatant of HTR8/SVneo cells were detected using ELISA (C). All experiments were repeated 3 times independently in identical conditions. Results are presented as mean ± SEM. Statistical comparison in separate groups between miR-210 and NC was performed using SPSS, with p<0.05 considered as significant. *p < 0.05, **p < 0.01, ***p < 0.001.