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Bacterial strain, plasmids, and primers used in the study
| Strain, plasmid, or primer | Genotype or sequence | Source or reference |
|---|---|---|
| E. coli TOP10F′ | Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen (NO-C615-00) |
| Plasmids | ||
| pSP-luc | Ampr Ori colE1 | Promega (E1781) |
| pRB474 | Ampr Cmr Ori− Ori+ | 6 |
| Primers | ||
| LucF (5′-3′) | AGG AAG GAT CCA GAG CAG ATT GTA CTG AGA Ga | This study |
| LucR (5′-3′) | AAT GCA GGT TAA CCT GGC TTA TCG AAA TT |
Underlined nucleotides show the position of the BamHI restriction site.
