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. 2016 Dec 9;11(12):e0167108. doi: 10.1371/journal.pone.0167108

Fig 1. Workflow of the experimental protocol.

Fig 1

C (control group, did not exercise); E = only conditioning; Car = conditioning plus L-carnitine supplementation; Chr = conditioning plus chromium supplementation; ET (exercise total: all fillies undergoing the conditioning were analyzed as a separate treatment). IET, incremental exercise test (IET-1 and IET-2, before and after aerobic conditioning, respectively); VLT (velocity related to lactate threshold) was used as a guide to determine the training intensity, 70–80% VLT; fs, fecal sample; mb, muscle biomarkers (plasma lactate and pH, CK and AST). Fatigue in the IETs was identified by the fillies’ inability to follow treadmill speed.