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. 2016 Dec 9;11(12):e0166948. doi: 10.1371/journal.pone.0166948

Fig 4.

Fig 4

Effect of CT0 on F508delCFTR-HEK and CFBE epithelial cell chloride transport measured by FLIPR assay A) Fluorescence measurement (relative fluorescent units, chloride transport surrogate) of quadruplicate F508delCFTR-HEK cell cultures maintained at 27°C for 4hr,○-○ untreated control F508delCFTR cells, □-□ Wt CFTR cells, ◇-◇ 3μM VX809 treated F508delCFTR cells, ●-● 65pM CT0 treated F508delCFTR cells, ▲⋯▲ 260pM CT0 treated F508delCFTR cells. Mean of 4 replicate cultures is shown ±SD. 10μM Forskolin, 100nM VX770 activator and 20μM CFTR inhibitor 172 were added to all cultures at the time marked by the arrows. B) Quadruplicate CFBE cell cultures at 37°C ± 2hr treatment, ■___■untreated control F508delCFTR cells ●-∙-∙● 3μM VX809 treated F508delCFTR cells, ◆_ _ _◆130pM CT0 treated F508delCFTR cells, ▲⋯▲13pM CT0 treated F508delCFTR cells. Mean ±SD. C) Quadruplicate CFBE cell cultures at 37°C ± 4hr treatment, ■__■ untreated control, ●-∙-∙● 3μM VX809 treated cells, ◆_ _ _◆130pM CT0 treated cells, ▲⋯▲13pM CT0 treated cells. Mean ±SD. Arrows in B and C mark 10μM forskolin addition.